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KMID : 0368420080510010025
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Journal of Plant Biology
2008 Volume.51 No. 1 p.25 ~ p.34
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Overexpression of aThellungiella halophila CBl9 homolog,ThCBL9, confers salt and osmotic tolerances in transgenicarabidopsis thaliana
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Sun Zhibin
Qi Xingyun
Li Pinghua
Wu Chunxia
Zhao Yanxiu
Zhang Hui
Wang Zenglan
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Abstract
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The calcineurin B-like (CBL) proteins, comprising a large subfamily of calcium sensors in plant cells, play an important role in many stress responses. We cloned a gene from the halophyteThellungiella halophila that is homologous toAtCBL9 inArabidopsis thaliana. The 1008-bpThCBL9 contains an ORF of 639 bp and encodes 213 amino acids, with a 5“-untranslated region of 193 bp and a 3”-untranslated region of 176 bp. Its amino acid sequence shares high homology with AtCBLs.ThCBL9 is up-regulated by ABA, NaCI, and PEG inThellungiella leaves. Using molecular biological methods, we over-expressedThCBL9 inA. thaliana and found that this enhanced tolerances to both high salt and osmotic stress in transgenicArabidopsis.
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KEYWORD
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Overexpression, stress tolerance, ThCBL9, Thellungiella halophila
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